Staining:Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering.
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal antiGAPDH (1:10.000, Abcam), mouse monoclonal antivinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Heterogeneous potential of human airway basal cells: Holoclone-stem cell identification in a clinical-grade system
Article Snippet: Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-Vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), mouse monoclonal anti-Actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.
Expressing:Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering.
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal antiGAPDH (1:10.000, Abcam), mouse monoclonal antivinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Heterogeneous potential of human airway basal cells: Holoclone-stem cell identification in a clinical-grade system
Article Snippet: Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-Vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), mouse monoclonal anti-Actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.
Two Tailed Test:Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering.
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal antiGAPDH (1:10.000, Abcam), mouse monoclonal antivinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Heterogeneous potential of human airway basal cells: Holoclone-stem cell identification in a clinical-grade system
Article Snippet: Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-Vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), mouse monoclonal anti-Actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.
Clone Assay:Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering.
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal antiGAPDH (1:10.000, Abcam), mouse monoclonal antivinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Airway epithelial stem cell renewal and differentiation: overcoming challenging steps towards clinical-grade tissue engineering
Article Snippet: Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.Protein band immunoreactions were performed with different primary antibodies (Supplementary Table 2) diluted in blocking solution, which were added to the membranes overnight at 4 °C.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), and mouse monoclonal anti-actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.The corresponding HRP-conjugated secondary antibodies (Supplementary Table 2) were diluted in blocking solution and incubated for 1 h at RT after three washes with 1× PBS.
Article Title: Heterogeneous potential of human airway basal cells: Holoclone-stem cell identification in a clinical-grade system
Article Snippet: Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.Protein bands immunoreactions were performed with different primary antibodies ( Key resource table ) diluted in the blocking solution, added overnight at 4 °C to the membranes.. The following primary antibodies were used: rabbit polyclonal anti-p63-alpha (1:5000, customized), rabbit monoclonal anti-BMI1 (1:500, Cell Signalling Technology), rabbit monoclonal anti-SOX2 (1:200, Cell Signalling Technology), mouse monoclonal anti-GAPDH (1:10.000, Abcam), mouse monoclonal anti-Vinculin (1:10.000, Sigma-Aldrich), rabbit polyclonal anti-KERATIN 14 (1:40.000, Biolegend), mouse monoclonal anti-Involucrin (1:10.000, Leica Microsystems), mouse monoclonal anti-Actin (1:5000, Abcam).. The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.The corresponding HRP-conjugated secondary antibodies ( Key resource table ) were diluted in the blocking solution and incubated for 1 hour at RT after three washings with 1x PBS.
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